Nau'o'i 15 na Babban Haɗarin Mutum Papillomavirus E6/E7 Gene mRNA
Sunan samfur
HWTS-CC005A-15 Nau'in Babban Haɗarin Mutum Papillomavirus E6/E7 Gene mRNA Gane Kit (Fluorescence PCR)
Epidemiology
Ciwon daji na mahaifa yana daya daga cikin nau'in ciwon daji na mata da aka fi sani a duk duniya, kuma faruwarsa yana da alaƙa da ƙwayoyin cuta na papillomavirus (HPV), amma kaɗan ne kawai na kamuwa da cutar HPV ke iya haɓaka zuwa kansa.Babban haɗarin HPV yana cutar da ƙwayoyin epithelial na mahaifa kuma yana samar da oncoproteins guda biyu, E6 da E7.Wannan furotin zai iya rinjayar nau'ikan sunadaran salula (irin su furotin suppressor proteins pRB da p53), tsawaita zagayowar tantanin halitta, yana shafar haɗin DNA da kwanciyar hankali na kwayoyin halitta, kuma yana tsoma baki tare da martanin rigakafin rigakafi da antitumor.
Tashoshi
| Tashoshi | Bangaren | An gwada Genotype |
| FAM | HPV Reaction Buffer 1 | HPV16, 31, 33, 35, 51, 52, 58 |
| VIC/HEX | Halin β-actin na mutum | |
| FAM | HPV Reaction Buffer 2 | HPV 18, 39, 45, 53, 56, 59, 66, 68 |
| VIC/HEX | Human INS gene |
Ma'aunin Fasaha
| Adana | Ruwa: ≤-18℃ |
| Rayuwar rayuwa | watanni 9 |
| Nau'in Samfura | kwayar exfoliated ta mahaifa |
| Ct | ≤38 |
| CV | ≤5.0% |
| LoD | 500 Kwafi/ml |
| Abubuwan da ake Aiwatar da su | Aiwatar da Tsarin Halitta 7500 Tsarin PCR na Gaskiya Abubuwan da aka Aiwatar da Tsarin Halittu 7500 Tsarin PCR Mai Saurin Real-Time QuantStudio®5 Tsarin PCR na Gaskiya SLAN-96P Tsarin PCR na Gaskiya LightCycler®480 Tsarin PCR na Gaskiya LineGene 9600 Plus Tsarin Gane PCR na Gaskiya MA-6000 Mai ƙididdige yawan zafin jiki na ainihi BioRad CFX96 Tsarin PCR na Gaskiya BioRad CFX Opus 96 Tsarin PCR na Gaskiya |
Gudun Aiki
Shawarar hakar reagent: Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3020-50-HPV15) ta Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. A hakar ya kamata a gudanar sosai bisa ga umarnin don amfani. .Adadin da aka ba da shawarar shine 50μL.Idan samfurin bai gama narkewa ba, mayar da shi zuwa mataki na 4 don sake narkewa.Sannan gwada bisa ga umarnin don amfani.
Nasihar hakar reagent: RNAprep Pure Animal Tissue Total RNA Extraction Kit (DP431).Ya kamata a gudanar da hakar bisa ga umarnin don amfani sosai (A cikin mataki na 5, ninka maida hankali na maganin aiki na DNaseI, wato, ɗauki 20μL na RNase-Free DNaseI (1500U) cikin sabon bututu mai kyauta na RNase-Free, ƙara 60μL na RDD buffer, kuma ku gauraya a hankali).Adadin da aka ba da shawarar shine 60μL.Idan samfurin bai gama narkewa ba, mayar da shi zuwa mataki na 5 don sake narkewa.Sannan gwada bisa ga umarnin don amfani.
