Coxsackie Virus Type A16 Nucleic Acid

Wannan kit ɗin yana ɗaukar Enzymatic Probe Isothermal Amplification (EPIA), kuma yana ƙirƙira ƙayyadaddun ƙayyadaddun ƙayyadaddun ƙayyadaddun bayanai da RNA tushen bincike (rProbe) don yankin da aka kiyaye sosai na Cox A16, kuma yana ƙara Bst enzyme da RNaseH enzyme a lokaci guda, wanda hagu da hagu. Ƙarshen dama na tushe na RNA na rProbe suna da lakabi tare da ƙungiyoyi masu kyalli da quencher, bi da bi.Yi amfani da aikin DNA polymerase da aikin maye gurbi na Bst enzyme don haɓaka makasudin da za a gwada shi a matsakaicin zafin jiki, RNaseH enzyme zai iya raba tushen RNA akan sarkar-bincike matasan, ta yadda ƙungiyar masu kyalli da masu kashe rProbe su ne. rabu da shi fluorescing.Bugu da kari, za a iya amfani da guntun hagu na ragowar rProbe RNA tushe don ƙara haɓaka samfuri, wanda ke ƙara tara samfurin.Ana ci gaba da tara siginar mai kyalli tare da samuwar samfurin, ta haka ne aka gano ainihin acid nucleic.