Human Papillomavirus (Nau'i 28) Kit ɗin Gano Ganewa (Fluorescence PCR)

Takaitaccen Bayani:

Ana amfani da wannan kit ɗin don gano ƙimar nucleic acid na nau'ikan papillomavirus na mutum 28 (HPV6, 11, 16, 18, 26, 31, 33, 35, 39, 40, 42, 43, 44, 45, 51,5). .


Cikakken Bayani

Tags samfurin

Sunan samfur

HWTS-CC004A-Human Papillomavirus (Nau'i 28) Kayan Gane Genotyping (Fluorescence PCR)

Epidemiology

Kit ɗin yana amfani da hanyar gano hasken walƙiya da yawa (PCR).An ƙirƙira ƙayyadaddun ƙayyadaddun ƙayyadaddun bayanai da bincike bisa tsarin L1 na manufa na HPV.An yi wa takamaiman binciken da FAM fluorophore (HPV6, 16, 26, 40, 53, 58, 73), VIC/HEX fluorophore (HPV11, 18, 33, 43, 51, 59, 81), CY5 fluorophore (HPV35, 4). , 45, 54, 56, 68, 82) da ROX fluorophore (HPV31, 39, 42, 52, 61, 66, 83) a 5', kuma 3' quencher rukuni shine BHQ1 ko BHQ2.A lokacin haɓakawa na PCR, ƙayyadaddun firamare da bincike suna ɗaure ga jerin abubuwan da suke niyya.Lokacin da Taq enzyme ya ci karo da binciken da ke daure zuwa jerin abubuwan da aka yi niyya, yana aiwatar da aikin 5 'karshen exonuclease don raba mai ba da rahoto fluorophore daga quencher fluorophore, ta yadda tsarin kula da hasken wuta zai iya karɓar siginar kyalli, wato, duk lokacin da DNA. An haɓaka madaidaicin, an samar da kwayar halitta mai kyalli, wanda ke fahimtar cikakkiyar daidaitawar tarin siginar kyalli da kuma samar da samfuran PCR, don cimma ƙimar inganci da ƙirar genotyping na acid nucleic na nau'ikan papillomavirus na mutum 28 a cikin samfuran sel exfoliated na mahaifa. .

Tashoshi

FAM

16,58,53,73,6,26,40.

VIC/HEX

18,33,51,59,11,81,43

ROX 31,66,52,39,83,61,42
CY5 56,35,45,68,54,44,82

Ma'aunin Fasaha

Adanawa ≤-18 ℃ A cikin duhu
Rayuwar rayuwa

watanni 12

Nau'in Samfura Kwayoyin exfoliated Cervical
Ct ≤25
CV

≤5.0%

LoD

Kwafi 25 / amsawa

Abubuwan da ake Aiwatar da su Sauƙaƙan Tsarin Ganewar Fluorescence na Gaskiya na Amp (HWTS1600)

 

Abubuwan da aka Aiwatar da Biosystems 7500 Tsarin PCR na Gaskiya

 

Abubuwan da aka Aiwatar da Tsarin Halittu 7500 Tsarin PCR Mai Saurin Real-Time

 

QuantStudio®5 Tsarin PCR na Gaskiya

 

SLAN-96P Tsarin PCR na Gaskiya

 

LightCycler®480 Tsarin PCR na Gaskiya

 

LineGene 9600 Plus Tsarin Gane PCR na Gaskiya

 

MA-6000 Mai ƙididdige yawan zafin jiki na ainihi

 

BioRad CFX96 Tsarin PCR na Gaskiya

 

BioRad CFX Opus 96 Tsarin PCR na Gaskiya

Gudun Aiki

Zabin 1.

Nasihar hakar reagent: Macro & Micro-Test Samfurin Sakin Reagent (HWTS-3005-8).

Zabin 2.

Shawarar hakar reagent: Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3004-32, HWTS-3004-48, HWTS-3004-96) da Macro & Micro-Test Atomatik Nucleic Acid Extractor (HWTS-3006).


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