MTHFR Gene Polymorphic Nucleic Acid
Sunan samfur
HWTS-GE004-MTHFR Gene Polymorphic Nucleic Acid Gane Kit (ARMS-PCR)
Epidemiology
Folic acid bitamin ne mai narkewa da ruwa wanda shine muhimmin cofactor a cikin hanyoyin rayuwa na jiki.A cikin 'yan shekarun nan, yawancin bincike sun tabbatar da cewa, maye gurbi na folate metabolizing enzyme gene MTHFR zai haifar da rashi na folic acid a cikin jiki, da kuma lalacewa na yau da kullum na ƙarancin folic acid a cikin manya na iya haifar da anemia megaloblastic, jijiyoyin jini. lalacewar endothelial, da dai sauransu. Rashin Folic acid a cikin mata masu juna biyu ba zai iya biyan bukatun kansu da na tayin ba, wanda zai iya haifar da lahani na jijiyoyi, anencephaly, haihuwa, da zubar da ciki.Matakan folate na jini suna shafar 5,10-methylenetetrahydrofolate reductase (MTHFR) polymorphisms.Maye gurbin 677C>T da 1298A>C a cikin kwayar halittar MTHFR suna haifar da jujjuyawar alanine zuwa valine da glutamic acid, bi da bi, yana haifar da raguwar ayyukan MTHFR kuma saboda haka rage amfani da folic acid.
Tashoshi
| FAM | Saukewa: MTHFR C677T |
| ROX | Saukewa: MTHFR A1298C |
| VIC(HEX) | Ikon cikin gida |
Ma'aunin Fasaha
| Adana | ≤-18℃ |
| Rayuwar rayuwa | watanni 12 |
| Nau'in Samfura | EDTA da aka tattara sabo-sassun jini mai maganin maƙarƙashiya |
| CV | ≤5.0% |
| Ct | ≤38 |
| LoD | 1.0ng/μL |
| Kayayyakin aiki: | Abubuwan da aka Aiwatar da Biosystems 7500 Tsarin PCR na Gaskiya Abubuwan da aka Aiwatar da Tsarin Halittu 7500 Tsarin PCR Mai Saurin Real-Time SLAN ®-96P Tsarukan PCR na Gaskiya QuantStudio™ 5 Tsarin PCR na Gaskiya LightCycler®480 Tsarin PCR na Gaskiya LineGene 9600 Plus Tsarin Gane PCR na Gaskiya MA-6000 Mai ƙididdige yawan zafin jiki na ainihi BioRad CFX96 Tsarin PCR na Gaskiya BioRad CFX Opus 96 Tsarin PCR na Gaskiya |
Gudun Aiki
Zabin 1
Abubuwan da aka ba da shawarar cirewa: Macro & Micro-Test Genomic DNA Kit (HWTS-3014-32, HWTS-3014-48, HWTS-3014-96) da Macro & Micro-Test Atomatik Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B) .
Zabin 2
Abubuwan da aka ba da shawarar hakar reagents: Kit ɗin Haɗin DNA na Jini (YDP348, JCXB20210062) na Tiangen Biotech(Beijing) Co., Ltd.Kit ɗin Haɓakar Jini (A1120) na Promega.
