Mutum BRAF Gene V600E Mutation
Sunan samfur
HWTS-TM007-Human BRAF Gene V600E Kayan Gane Maɓalli (Fluorescence PCR)
Takaddun shaida
CE
Epidemiology
Sama da nau'ikan maye gurbi na BRAF guda 30 aka samu, wanda kusan kashi 90% na cikin exon 15, inda ake ganin maye gurbin V600E shine maye gurbi na yau da kullun, wato thymine (T) a matsayi na 1799 a exon 15 an canza shi zuwa adenine (A), wanda ya haifar da maye gurbin valine (V) a matsayi na 600 ta hanyar glutamic acid (E) a cikin samfurin furotin.Ana samun maye gurbi na BRAF a cikin muggan ciwace-ciwace irin su melanoma, cancer colorectal, cancer thyroid, da ciwon huhu.Fahimtar maye gurbin kwayar halittar BRAF ya zama buƙatar tantance EGFR-TKIs da magungunan maye gurbi na BRAF a cikin magungunan da aka yi niyya na asibiti ga marasa lafiya waɗanda za su iya amfana.
Tashoshi
| FAM | maye gurbin V600E, kulawar ciki |
Ma'aunin Fasaha
| Adana | ≤-18℃ |
| Rayuwar rayuwa | watanni 9 |
| Nau'in Samfura | paraffin-inbedded pathological nama samfurori |
| CV | 5.0% |
| Ct | ≤38 |
| LoD | Yi amfani da kayan aikin don gano madaidaicin ingancin ingancin LoD.a) ƙarƙashin nau'in nau'in daji na 3ng / μL, ana iya gano ƙimar maye gurbin 1% a cikin buffer mai ƙarfi;b) ƙarƙashin 1% maye gurbi, maye gurbi na 1 × 103Kwafi/ml a cikin nau'in daji na 1 × 105Ana iya gano kwafi/ml a tabbatattu a cikin buffer dauki;c) IC Reaction Buffer na iya gano mafi ƙasƙanci gano iyaka ingancin iko SW3 na kamfani na cikin gida. |
| Kayayyakin aiki: | Abubuwan da aka Aiwatar da Biosystems 7500 Tsarin PCR na GaskiyaAiwatar da Biosystems 7300 Real-Time PCR Tsarukan, QuantStudio® 5 Tsarin PCR na Gaskiya LightCycler®480 Tsarin PCR na Gaskiya BioRad CFX96 Tsarin PCR na Gaskiya |
Gudun Aiki
Abubuwan da aka ba da shawarar cirewa: QIAGEN's QIAamp DNA FFPE Tissue Kit (56404), Kayan aikin Tissue DNA Rapid Extraction Kit (DP330) wanda Tiangen Biotech(Beijing) Co., Ltd ke ƙera.
