Mutum BRAF Gene V600E Mutation

Takaitaccen Bayani:

Ana amfani da wannan kayan gwajin don gano ainihin maye gurbi na BRAF gene V600E a cikin samfuran nama da aka haɗa da paraffin na melanoma na ɗan adam, ciwon daji na launi, ciwon thyroid da kansar huhu a cikin vitro.


Cikakken Bayani

Tags samfurin

Sunan samfur

HWTS-TM007-Human BRAF Gene V600E Kayan Gane Maɓalli (Fluorescence PCR)

Takaddun shaida

CE

Epidemiology

Sama da nau'ikan maye gurbi na BRAF guda 30 aka samu, wanda kusan kashi 90% na cikin exon 15, inda ake ganin maye gurbin V600E shine maye gurbi na yau da kullun, wato thymine (T) a matsayi na 1799 a exon 15 an canza shi zuwa adenine (A), wanda ya haifar da maye gurbin valine (V) a matsayi na 600 ta hanyar glutamic acid (E) a cikin samfurin furotin.Ana samun maye gurbi na BRAF a cikin muggan ciwace-ciwace irin su melanoma, cancer colorectal, cancer thyroid, da ciwon huhu.Fahimtar maye gurbin kwayar halittar BRAF ya zama buƙatar tantance EGFR-TKIs da magungunan maye gurbi na BRAF a cikin magungunan da aka yi niyya na asibiti ga marasa lafiya waɗanda za su iya amfana.

Tashoshi

FAM maye gurbin V600E, kulawar ciki

Ma'aunin Fasaha

Adana

≤-18℃

Rayuwar rayuwa

watanni 9

Nau'in Samfura

paraffin-inbedded pathological nama samfurori

CV

5.0%

Ct

≤38

LoD

Yi amfani da kayan aikin don gano madaidaicin ingancin ingancin LoD.a) ƙarƙashin nau'in nau'in daji na 3ng / μL, ana iya gano ƙimar maye gurbin 1% a cikin buffer mai ƙarfi;b) ƙarƙashin 1% maye gurbi, maye gurbi na 1 × 103Kwafi/ml a cikin nau'in daji na 1 × 105Ana iya gano kwafi/ml a tabbatattu a cikin buffer dauki;c) IC Reaction Buffer na iya gano mafi ƙasƙanci gano iyaka ingancin iko SW3 na kamfani na cikin gida.

Kayayyakin aiki:

Abubuwan da aka Aiwatar da Biosystems 7500 Tsarin PCR na GaskiyaAiwatar da Biosystems 7300 Real-Time PCR

Tsarukan, QuantStudio® 5 Tsarin PCR na Gaskiya

LightCycler®480 Tsarin PCR na Gaskiya

BioRad CFX96 Tsarin PCR na Gaskiya

Gudun Aiki

Abubuwan da aka ba da shawarar cirewa: QIAGEN's QIAamp DNA FFPE Tissue Kit (56404), Kayan aikin Tissue DNA Rapid Extraction Kit (DP330) wanda Tiangen Biotech(Beijing) Co., Ltd ke ƙera.


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