Mutum CYP2C9 da VKORC1 Gene Polymorphism
Sunan samfur
HWTS-GE014A-Human CYP2C9 da VKORC1 Gene Polymorphism Gane Kit (Fluorescence PCR)
Takaddun shaida
CE
Epidemiology
Warfarin maganin maganin jini ne na baka wanda aka saba amfani dashi a aikin asibiti a halin yanzu, wanda aka yi niyya don rigakafi da magance cututtukan thromboembolic.Koyaya, warfarin yana da ƙayyadaddun taga magani kuma ya bambanta sosai tsakanin jinsi da daidaikun mutane daban-daban.Ƙididdiga sun nuna cewa bambancin barga a cikin mutane daban-daban na iya zama fiye da sau 20.Zubar da jini mara kyau yana faruwa a cikin 15.2% na marasa lafiya da ke shan warfarin kowace shekara, wanda kashi 3.5% ke haifar da zubar da jini mai mutuwa.Nazarin Pharmacogenomic sun nuna cewa polymorphism na kwayoyin halitta na manufa enzyme VKORC1 da kuma enzyme na rayuwa CYP2C9 na warfarin wani muhimmin abu ne wanda ke shafar bambancin kashi na warfarin.Warfarin shine takamaiman mai hana bitamin K epoxide reductase (VKORC1), don haka yana hana haɓakar abubuwan da ke tattare da ƙwayar cuta da ke tattare da bitamin K kuma yana ba da maganin hana jini.Yawancin karatu sun nuna cewa polymorphism na kwayar halitta na VKORC1 mai gabatarwa shine mafi mahimmancin abin da ya shafi tseren da bambance-bambancen mutum a cikin adadin da ake buƙata na warfarin.CYP2C9 ne ke daidaita Warfarin, kuma kwayoyin halittarsa suna rage saurin warfarin.Mutanen da ke amfani da warfarin suna da haɗari mafi girma (sau biyu zuwa uku) na zubar jini a farkon matakin amfani.
Tashoshi
| FAM | VKORC1 (-1639G>A) |
| CY5 | CYP2C9*3 |
| VIC/HEX | IC |
Ma'aunin Fasaha
| Adana | Ruwa: ≤-18℃ |
| Rayuwar rayuwa | watanni 12 |
| Nau'in Samfura | Fresh EDTA anticoagulated jini |
| CV | ≤5.0% |
| LoD | 1.0ng/μL |
| Musamman | Babu wani giciye-reactivity tare da wasu daidaitattun jerin kwayoyin halittar ɗan adam (jinin CYP2C19 ɗan adam, ɗan adam RPN2 gene);maye gurbin CYP2C9*13 da VKORC1 (3730G>A) a waje da kewayon gano wannan kit ɗin. |
| Abubuwan da ake Aiwatar da su | Abubuwan da aka Aiwatar da Biosystems 7500 Tsarin PCR na Gaskiya Abubuwan da aka Aiwatar da Tsarin Halittu 7500 Tsarin PCR Mai Saurin Real-Time QuantStudio®5 Tsarin PCR na Gaskiya SLAN-96P Tsarin PCR na Gaskiya LightCycler®480 Tsarin PCR na Gaskiya LineGene 9600 Plus Tsarin Gane PCR na Gaskiya MA-6000 Mai ƙididdige yawan zafin jiki na ainihi BioRad CFX96 Tsarin PCR na Gaskiya BioRad CFX Opus 96 Tsarin PCR na Gaskiya |
Gudun Aiki
Shawarar hakar reagent: Macro & Micro-Test Viral DNA/RNA Kit(HWTS-3001, HWTS-3004-32, HWTS-3004-48, HWTS-3004-96) da Macro & Micro-Test Atomatik Nucleic Acid Extractor(HWTS- 3006).
