Mutum Methylated NDRG4/SEPT9/SFRP2/BMP3/SDC2 Gene

Takaitaccen Bayani:

An yi nufin kit ɗin don gano ƙimar ingancin methylated NDRG4/SEPT9/SFRP2/BMP3/SDC2 a cikin ƙwayoyin exfoliated na hanji a cikin samfuran stool na ɗan adam.


Cikakken Bayani

Tags samfurin

Sunan samfur

HWTS-OT077-Human Methylated NDRG4/SEPT9/SFRP2/BMP3/SDC2 Kayan Gano Gene (Fluorescence PCR)

Takaddun shaida

CE

Epidemiology

A cikin manya, ƙwayoyin epithelial na hanji sama da 10 8 suna faɗowa daga bangon hanji kowace rana, kuma ana fitar da su tare da najasa ta hanyar babban peristalsis na hanji.Saboda ƙwayoyin tumor suna iya faɗuwa daga sashin hanji na haɓakar da ba a saba ba, stool na marasa lafiya na hanji yana ƙunshe da ƙwayoyin cuta da yawa da abubuwan da ba su da kyau, wanda shine tushen kayan aiki don gano kwanciyar hankali.Nazarin ya gano cewa gyare-gyaren methylation na masu tallata kwayoyin halitta wani abu ne na farko a cikin tumorigenesis, kuma kwayoyin halitta da aka samo daga samfurori na masu ciwon daji na launin fata na iya nuna kasancewar ciwon daji a cikin hanji a baya.

NDRG4, wanda aka fi sani da SMAP-8 da BDM1, yana ɗaya daga cikin mambobi huɗu na dangin NDRG (NDRG1-4), wanda aka nuna yana da alaƙa da yaduwar kwayar halitta, bambance-bambance, ci gaba da damuwa.An tabbatar da cewa NDRG4 methylation wata alama ce mai yuwuwar biomarker don gano cutar kansa mara lalacewa a cikin samfuran stool.

SEPT9 memba ne na dangin halittar Septin, wanda ya ƙunshi akalla 13 kwayoyin halitta waɗanda ke ɓoye yankin GTPase da aka kiyaye wanda zai iya ɗaure sunadaran da ke da alaƙa da cytoskeleton, kuma yana da alaƙa da rabon tantanin halitta da ƙari.Nazarin ya gano cewa abun ciki na methylated Septin9 gene yana haɓaka haɓaka a cikin samfuran stool daga marasa lafiya da ciwon daji.

Sunadaran da ke da alaƙa da ɓoye (sFRPs) sunadaran sunadarai masu narkewa waɗanda rukuni ne na masu adawa da hanyar Wnt saboda babban tsarin tsarin su ga mai karɓar frizzled (Fz) don siginar Wnt.Rashin kunna kwayar halittar SFRP yana haifar da kunnawar siginar Wnt marar sarrafawa wanda ke da alaƙa da ciwon daji na launi.A halin yanzu, SFRP2 methylation a cikin stool za a iya amfani da shi azaman biomarker mara lalacewa don ganewar ciwon daji na colorectal.

BMP3 memba ne na TGF-B superfamily kuma don haka yana taka muhimmiyar rawa wajen ci gaban amfrayo ta hanyar haifar da siffata farkon samuwar kashi.BMP3 yana da hypermethylated a cikin ciwon daji na colorectal kuma ana iya amfani dashi azaman alamar ƙari mai mahimmanci.

SDC2 shine tantanin halitta heparan sulfate proteoglycan wanda ke da hannu a cikin tsari na yawancin hanyoyin physiological da pathological.Abubuwan da aka sarrafa na jiki sun haɗa da yaduwar kwayar halitta, bambance-bambance, adhesion, ƙungiyar cytoskeletal, ƙaura, warkar da rauni, sadarwar cell-matrix, angiogenesis;Hanyoyin cututtuka sun haɗa da kumburi da ciwon daji.Matsayin methylation na SDC2 gene a cikin kyallen daji na launin launi ya fi girma fiye da na kyallen takarda na yau da kullun.

Tashoshi

Reaction buffer A

VIC/HEX methylated NDRG4 gene
ROX methylated SEPT9 gene
CY5 kula da ciki

Mai ɗaukar martani B

VIC/HEX methylated SFRP2 gene
ROX methylated BMP3 gene
FAM methylated SDC2 gene
CY5 kula da ciki

Tafsiri

Gene

Tashar siginar

Ct Darajar

Tafsiri

NDRG4

VIC (HEX)

Ct darajar≤38

NDRG4 tabbatacce

Ct darajar> 38 ko unde

NDRG4 korau

SEPT9

ROX

Ct darajar≤38

SEPT9 tabbatacce

Ct darajar> 38 ko unde

SEPT9 mara kyau

Farashin SFRP2

VIC (HEX)

Ct darajar≤38

SFRP2 tabbatacce

Ct darajar> 38 ko unde

Farashin SFRP2

Ma'aunin Fasaha

Adana Ruwa: ≤-18℃
Rayuwar rayuwa watanni 9
Nau'in Samfura Samfurin Stool
CV ≤5.0%
Musamman Babu giciye-reactivity tare da ciwon hanta, ciwon bile duct cancer, thyroid ciwon daji da kuma huhu ciwon daji
Abubuwan da ake Aiwatar da su QuantStudio ®5 Tsarin PCR na Gaskiya

SLAN-96P Tsarin PCR na Gaskiya

LightCycler®480 Tsarin PCR na Gaskiya

LineGene 9600 Plus Tsarin Gane PCR na Gaskiya

MA-6000 Mai ƙididdige yawan zafin jiki na ainihi

Gudun Aiki

Shawarar hakar reagent: Macro & Micro-Test Viral DNA/RNA Kit(HWTS-3001, HWTS-3004-32, HWTS-3004-48, HWTS-3004-96) da Macro & Micro-Test Atomatik Nucleic Acid Extractor(HWTS- 3006).


  • Na baya:
  • Na gaba:

  • Ku rubuta sakonku anan ku aiko mana