Human Papillomavirus (Nau'i 28) Kit ɗin Gano Ganewa (Fluorescence PCR)

Kit ɗin yana amfani da hanyar gano hasken walƙiya da yawa (PCR).An ƙirƙira ƙayyadaddun ƙayyadaddun ƙayyadaddun bayanai da bincike bisa tsarin L1 na manufa na HPV.An yi wa takamaiman binciken da FAM fluorophore (HPV6, 16, 26, 40, 53, 58, 73), VIC/HEX fluorophore (HPV11, 18, 33, 43, 51, 59, 81), CY5 fluorophore (HPV35, 4). , 45, 54, 56, 68, 82) da ROX fluorophore (HPV31, 39, 42, 52, 61, 66, 83) a 5', kuma 3' quencher rukuni shine BHQ1 ko BHQ2.A lokacin haɓakawa na PCR, ƙayyadaddun firamare da bincike suna ɗaure ga jerin abubuwan da suke niyya.Lokacin da Taq enzyme ya ci karo da binciken da ke daure zuwa jerin abubuwan da aka yi niyya, yana aiwatar da aikin 5 'karshen exonuclease don raba mai ba da rahoto fluorophore daga quencher fluorophore, ta yadda tsarin kula da hasken wuta zai iya karɓar siginar kyalli, wato, duk lokacin da DNA. An haɓaka madaidaicin, an samar da kwayar halitta mai kyalli, wanda ke fahimtar cikakkiyar daidaitawar tarin siginar kyalli da kuma samar da samfuran PCR, don cimma ƙimar inganci da ƙirar genotyping na acid nucleic na nau'ikan papillomavirus na mutum 28 a cikin samfuran sel exfoliated na mahaifa. .