Mutum ROS1 Fusion Gene Mutation

Takaitaccen Bayani:

Ana amfani da wannan kit ɗin don gano in vitro qualitative gano nau'ikan 14 na ROS1 fusion gene maye gurbi a cikin samfuran cutar kansar huhun da ba ƙaramin cell ba (Table 1).Sakamakon gwajin don nunin asibiti ne kawai kuma bai kamata a yi amfani da shi a matsayin tushen kawai don maganin keɓantaccen mutum na marasa lafiya ba.


Cikakken Bayani

Tags samfurin

Sunan samfur

HWTS-TM009-Human ROS1 Fusion Gene Mutation Kit (Fluorescence PCR)

Takaddun shaida

CE

Epidemiology

ROS1 shine transmembrane tyrosine kinase na dangin mai karɓar insulin.ROS1 fusion gene an tabbatar da shi azaman wani muhimmin kwayar cutar kansar huhu mara ƙarami.A matsayin wakilin sabon nau'in nau'in kwayoyin halitta na musamman, abubuwan da suka faru na ROS1 fusion gene a NSCLC Kimanin 1% zuwa 2% ROS1 yawanci yana jurewa tsarin sake tsarawa a cikin exons 32, 34, 35 da 36. Bayan an hade shi da kwayoyin halitta irin su CD74, EZR, SLC34A2, da SDC4, zai ci gaba da kunna yankin ROS1 tyrosine kinase.ROS1 kinase da aka kunna ba daidai ba zai iya kunna hanyoyin siginar ƙasa kamar RAS / MAPK / ERK, PI3K / Akt / mTOR, da JAK3 / STAT3, don haka shiga cikin haɓakawa, bambance-bambance da metastasis na ƙwayoyin tumo, da haifar da ciwon daji.Daga cikin maye gurbi na ROS1, CD74-ROS1 yana da kusan kashi 42%, EZR na kusan kashi 15%, SLC34A2 yana da kusan kashi 12%, SDC4 yana da kusan kashi 7%.Nazarin ya nuna cewa wurin daurin ATP na yanki na catalytic na ROS1 kinase da kuma ATP-binding site na ALK kinase suna da homology na har zuwa 77%, don haka ALK tyrosine kinase ƙananan kwayoyin inhibitor crizotinib da sauransu suna da tasirin warkewa a fili. a cikin maganin NSCLC tare da maye gurbi na ROS1.Saboda haka, gano ROS1 fusion maye gurbi shine jigo da tushe don jagorantar amfani da magungunan crizotinib.

Tashoshi

FAM Reaction Buffer 1, 2, 3 da 4
VIC(HEX) Reaction Buffer 4

Ma'aunin Fasaha

Adana

≤-18℃

Rayuwar rayuwa

watanni 9

Nau'in Samfura

nama mai cutarwa na paraffin ko samfuran yanki

CV

5.0%

Ct

≤38

LoD

Wannan kit ɗin na iya gano maye gurbi kamar ƙasa da kwafi 20.

Kayayyakin aiki:

Abubuwan da aka Aiwatar da Biosystems 7500 Tsarin PCR na GaskiyaAbubuwan da aka Aiwatar da Tsarin Halittu 7500 Tsarin PCR Mai Saurin Real-Time

SLAN ®-96P Tsarukan PCR na Gaskiya

QuantStudio™ 5 Tsarin PCR na Gaskiya

LightCycler®480 Tsarin PCR na Gaskiya

LineGene 9600 Plus Tsarin Gane PCR na Gaskiya

MA-6000 Mai ƙididdige yawan zafin jiki na ainihi

BioRad CFX96 Tsarin PCR na Gaskiya

BioRad CFX Opus 96 Tsarin PCR na Gaskiya

Gudun Aiki

Shawarar hakar reagent: RNeasy FFPE Kit (73504) daga QIAGEN, Paraffin Embedded Tissue Section Total RNA Extraction Kit (DP439) daga Tiangen Biotech(Beijing) Co., Ltd.


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